Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices

Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equ...

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Detalles Bibliográficos
Autores principales: Giacomoni, Jessica, Habekost, Mette, Cepeda-Prado, Efrain, Mattsson, Bengt, Ottosson, Daniella Rylander, Parmar, Malin, Kajtez, Janko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9873499/
https://www.ncbi.nlm.nih.gov/pubmed/36853668
http://dx.doi.org/10.1016/j.xpro.2022.102041
Descripción
Sumario:Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also provide instructions for culturing of rat brain slices free floating on permeable culture inserts, maintained in regular CO(2) incubators, and handled only at media change.

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