Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices

Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equ...

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Detalles Bibliográficos
Autores principales: Giacomoni, Jessica, Habekost, Mette, Cepeda-Prado, Efrain, Mattsson, Bengt, Ottosson, Daniella Rylander, Parmar, Malin, Kajtez, Janko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9873499/
https://www.ncbi.nlm.nih.gov/pubmed/36853668
http://dx.doi.org/10.1016/j.xpro.2022.102041

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9873499/
https://www.ncbi.nlm.nih.gov/pubmed/36853668
http://dx.doi.org/10.1016/j.xpro.2022.102041

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