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Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices
Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equ...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9873499/ https://www.ncbi.nlm.nih.gov/pubmed/36853668 http://dx.doi.org/10.1016/j.xpro.2022.102041 |