Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices

Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equ...

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Autores principales: Giacomoni, Jessica, Habekost, Mette, Cepeda-Prado, Efrain, Mattsson, Bengt, Ottosson, Daniella Rylander, Parmar, Malin, Kajtez, Janko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9873499/
https://www.ncbi.nlm.nih.gov/pubmed/36853668
http://dx.doi.org/10.1016/j.xpro.2022.102041
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author Giacomoni, Jessica
Habekost, Mette
Cepeda-Prado, Efrain
Mattsson, Bengt
Ottosson, Daniella Rylander
Parmar, Malin
Kajtez, Janko
author_facet Giacomoni, Jessica
Habekost, Mette
Cepeda-Prado, Efrain
Mattsson, Bengt
Ottosson, Daniella Rylander
Parmar, Malin
Kajtez, Janko
author_sort Giacomoni, Jessica
collection PubMed
description Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also provide instructions for culturing of rat brain slices free floating on permeable culture inserts, maintained in regular CO(2) incubators, and handled only at media change.
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spelling pubmed-98734992023-01-26 Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices Giacomoni, Jessica Habekost, Mette Cepeda-Prado, Efrain Mattsson, Bengt Ottosson, Daniella Rylander Parmar, Malin Kajtez, Janko STAR Protoc Protocol Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also provide instructions for culturing of rat brain slices free floating on permeable culture inserts, maintained in regular CO(2) incubators, and handled only at media change. Elsevier 2023-01-18 /pmc/articles/PMC9873499/ /pubmed/36853668 http://dx.doi.org/10.1016/j.xpro.2022.102041 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Giacomoni, Jessica
Habekost, Mette
Cepeda-Prado, Efrain
Mattsson, Bengt
Ottosson, Daniella Rylander
Parmar, Malin
Kajtez, Janko
Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices
title Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices
title_full Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices
title_fullStr Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices
title_full_unstemmed Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices
title_short Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices
title_sort protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9873499/
https://www.ncbi.nlm.nih.gov/pubmed/36853668
http://dx.doi.org/10.1016/j.xpro.2022.102041
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