Isolation of nuclei from frozen human subcutaneous adipose tissue for full-length single-nuclei transcriptional profiling

Automated single-cell dispensing is incompatible with white adipose tissue (WAT) due to lipid-laden adipocytes. Single-nuclei RNA-Seq permits transcriptional profiling of all cells from WAT. Human WAT faces unique technical challenges in isolating nuclei compared to rodent tissue due to greater extr...

Descripción completa

Detalles Bibliográficos
Autores principales: Whytock, Katie L., Divoux, Adeline, Sun, Yifei, Hopf, Meghan, Yeo, Reichelle X., Pino, Maria F., Yu, GongXin, Smith, Steven R., Walsh, Martin J., Sparks, Lauren M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9876942/
https://www.ncbi.nlm.nih.gov/pubmed/36853719
http://dx.doi.org/10.1016/j.xpro.2023.102054

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9876942/
https://www.ncbi.nlm.nih.gov/pubmed/36853719
http://dx.doi.org/10.1016/j.xpro.2023.102054

Ejemplares similares