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Optimized protocols for chromatin immunoprecipitation of exogenously expressed epitope-tagged proteins

Chromatin immunoprecipitation (ChIP) assay is widely used for investigating the interaction between DNA and DNA-binding proteins such as transcription factors, co-factors, or chromatin-associated proteins. However, a successful ChIP assay largely depends on the quality of a ChIP-grade primary antibo...

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Detalles Bibliográficos
Autores principales: Fang, Wentong, Liao, Chengheng, Zhang, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9876949/
https://www.ncbi.nlm.nih.gov/pubmed/36853721
http://dx.doi.org/10.1016/j.xpro.2023.102050