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Live‐cell super‐resolution imaging of actin using LifeAct‐14 with a PAINT‐based approach

We present direct‐LIVE‐PAINT, an easy‐to‐implement approach for the nanoscopic imaging of protein structures in live cells using labeled binding peptides. We demonstrate the feasibility of direct‐LIVE‐PAINT with an actin‐binding peptide fused to EGFP, the location of which can be accurately determin...

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Detalles Bibliográficos
Autores principales: Bhaskar, Haresh, Kleinjan, Dirk‐Jan, Oi, Curran, Gidden, Zoe, Rosser, Susan J., Horrocks, Mathew H., Regan, Lynne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9878614/
https://www.ncbi.nlm.nih.gov/pubmed/36585831
http://dx.doi.org/10.1002/pro.4558