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PCDH17 gene promoter methylation status in a cohort of Egyptian women with epithelial ovarian cancer

BACKGROUND AND OBJECTIVE: Ovarian cancer is a leading cause of female mortality. Epigenetic changes occur in early stages of carcinogenesis and represent a marker for cancer diagnosis. Protocadherin 17 (PCDH17) is a tumor suppressor gene involved in cell adhesion and apoptosis. The methylation of PC...

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Autores principales: Elsharkawi, Sherif Mohamed, Elkaffash, Dalal, Moez, Pacint, El-Etreby, Nour, Sheta, Eman, Taleb, Raghda Saad Zaghloul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9878799/
https://www.ncbi.nlm.nih.gov/pubmed/36698136
http://dx.doi.org/10.1186/s12885-023-10549-3
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author Elsharkawi, Sherif Mohamed
Elkaffash, Dalal
Moez, Pacint
El-Etreby, Nour
Sheta, Eman
Taleb, Raghda Saad Zaghloul
author_facet Elsharkawi, Sherif Mohamed
Elkaffash, Dalal
Moez, Pacint
El-Etreby, Nour
Sheta, Eman
Taleb, Raghda Saad Zaghloul
author_sort Elsharkawi, Sherif Mohamed
collection PubMed
description BACKGROUND AND OBJECTIVE: Ovarian cancer is a leading cause of female mortality. Epigenetic changes occur in early stages of carcinogenesis and represent a marker for cancer diagnosis. Protocadherin 17 (PCDH17) is a tumor suppressor gene involved in cell adhesion and apoptosis. The methylation of PCDH17 gene promoter has been described in several cancers including ovarian cancer. The aim of the study was to compare the methylation status of PCDH17 gene promoter between females diagnosed with epithelial ovarian cancer and a control group composed of normal and benign ovarian lesions. METHODS: Fifty female subjects were included in our study (25 ovarian cancer patients and 25 controls). DNA was extracted from Formalin-Fixed Paraffin-Embedded (FFPE) tissues of the subjects. Methylation levels for six CpG sites in the PCDH17 gene promoter were assessed by pyrosequencing. RESULTS: The methylation levels at five out of six sites were significantly higher in females with epithelial ovarian cancer compared to the control group. Moreover, the same applies for the mean methylation level with p value 0.018. CONCLUSION: Methylation of PCDH17 gene promoter plays a role in ovarian carcinogenesis and can be used for diagnosis and early detection.
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spelling pubmed-98787992023-01-27 PCDH17 gene promoter methylation status in a cohort of Egyptian women with epithelial ovarian cancer Elsharkawi, Sherif Mohamed Elkaffash, Dalal Moez, Pacint El-Etreby, Nour Sheta, Eman Taleb, Raghda Saad Zaghloul BMC Cancer Research BACKGROUND AND OBJECTIVE: Ovarian cancer is a leading cause of female mortality. Epigenetic changes occur in early stages of carcinogenesis and represent a marker for cancer diagnosis. Protocadherin 17 (PCDH17) is a tumor suppressor gene involved in cell adhesion and apoptosis. The methylation of PCDH17 gene promoter has been described in several cancers including ovarian cancer. The aim of the study was to compare the methylation status of PCDH17 gene promoter between females diagnosed with epithelial ovarian cancer and a control group composed of normal and benign ovarian lesions. METHODS: Fifty female subjects were included in our study (25 ovarian cancer patients and 25 controls). DNA was extracted from Formalin-Fixed Paraffin-Embedded (FFPE) tissues of the subjects. Methylation levels for six CpG sites in the PCDH17 gene promoter were assessed by pyrosequencing. RESULTS: The methylation levels at five out of six sites were significantly higher in females with epithelial ovarian cancer compared to the control group. Moreover, the same applies for the mean methylation level with p value 0.018. CONCLUSION: Methylation of PCDH17 gene promoter plays a role in ovarian carcinogenesis and can be used for diagnosis and early detection. BioMed Central 2023-01-25 /pmc/articles/PMC9878799/ /pubmed/36698136 http://dx.doi.org/10.1186/s12885-023-10549-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Elsharkawi, Sherif Mohamed
Elkaffash, Dalal
Moez, Pacint
El-Etreby, Nour
Sheta, Eman
Taleb, Raghda Saad Zaghloul
PCDH17 gene promoter methylation status in a cohort of Egyptian women with epithelial ovarian cancer
title PCDH17 gene promoter methylation status in a cohort of Egyptian women with epithelial ovarian cancer
title_full PCDH17 gene promoter methylation status in a cohort of Egyptian women with epithelial ovarian cancer
title_fullStr PCDH17 gene promoter methylation status in a cohort of Egyptian women with epithelial ovarian cancer
title_full_unstemmed PCDH17 gene promoter methylation status in a cohort of Egyptian women with epithelial ovarian cancer
title_short PCDH17 gene promoter methylation status in a cohort of Egyptian women with epithelial ovarian cancer
title_sort pcdh17 gene promoter methylation status in a cohort of egyptian women with epithelial ovarian cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9878799/
https://www.ncbi.nlm.nih.gov/pubmed/36698136
http://dx.doi.org/10.1186/s12885-023-10549-3
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