Protocol using lentivirus to establish THP-1 suspension cell lines for immunostaining and confocal microscopy

THP-1, a monocyte cell line growing in suspension, is widely used in immunology research. However, establishing suspension cell lines and performing confocal microscopy can be challenging. Here, we present a protocol to efficiently generate THP-1 cell lines using lentivirus and perform immunostainin...

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Detalles Bibliográficos
Autores principales: Ji, Wangsheng, Zhang, Lianfei, Liu, Xinqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9879989/
https://www.ncbi.nlm.nih.gov/pubmed/36853734
http://dx.doi.org/10.1016/j.xpro.2022.102032

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9879989/
https://www.ncbi.nlm.nih.gov/pubmed/36853734
http://dx.doi.org/10.1016/j.xpro.2022.102032

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