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Protocol using lentivirus to establish THP-1 suspension cell lines for immunostaining and confocal microscopy
THP-1, a monocyte cell line growing in suspension, is widely used in immunology research. However, establishing suspension cell lines and performing confocal microscopy can be challenging. Here, we present a protocol to efficiently generate THP-1 cell lines using lentivirus and perform immunostainin...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9879989/ https://www.ncbi.nlm.nih.gov/pubmed/36853734 http://dx.doi.org/10.1016/j.xpro.2022.102032 |