Protocol for combining immunological procedures with cell cycle stage synchronization to detect cell-cycle-specific complexes

Here, we present a protocol combining co-immunoprecipitation (Co-IP) and immunofluorescence approaches with cell cycle stage synchronization to detect cell-cycle-specific complexes. We describe steps to synchronize cells at specific cell cycle stages using drugs. We then detail the preparation of ce...

Descripción completa

Detalles Bibliográficos
Autores principales: Habu, Toshiyuki, Kim, Jiyeong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9881401/
https://www.ncbi.nlm.nih.gov/pubmed/36853678
http://dx.doi.org/10.1016/j.xpro.2023.102063

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9881401/
https://www.ncbi.nlm.nih.gov/pubmed/36853678
http://dx.doi.org/10.1016/j.xpro.2023.102063

Ejemplares similares