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Protocol for combining immunological procedures with cell cycle stage synchronization to detect cell-cycle-specific complexes
Here, we present a protocol combining co-immunoprecipitation (Co-IP) and immunofluorescence approaches with cell cycle stage synchronization to detect cell-cycle-specific complexes. We describe steps to synchronize cells at specific cell cycle stages using drugs. We then detail the preparation of ce...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9881401/ https://www.ncbi.nlm.nih.gov/pubmed/36853678 http://dx.doi.org/10.1016/j.xpro.2023.102063 |