Click-chemistry-based protocol to quantitatively assess fatty acid uptake by Mycobacterium tuberculosis in axenic culture and inside mouse macrophages

Mycobacterium tuberculosis (Mtb) hijacks host-derived fatty acids (FAs) to sustain its intracellular growth inside host cells. Here, we present a click-chemistry-based protocol to assess FA import by Mtb in axenic culture or inside mouse macrophages. We describe the use of alkyne analogs of natural...

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Detalles Bibliográficos
Autores principales: Laval, Thomas, Demangel, Caroline
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9881404/
https://www.ncbi.nlm.nih.gov/pubmed/36853675
http://dx.doi.org/10.1016/j.xpro.2023.102062

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9881404/
https://www.ncbi.nlm.nih.gov/pubmed/36853675
http://dx.doi.org/10.1016/j.xpro.2023.102062

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