Identification of newly translated thermo-sensitive proteins using pulse SILAC mass spectrometry and the GAL promoter system

Some newly translated proteins are more susceptible to misfolding and aggregation upon heat shock in comparison to other proteins. To study these newly translated thermo-sensitive proteins on a proteomic scale, we present here a protocol that combines pulse-SILAC with biochemical fractionation for m...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhu, Mang, Calabrese, Gaetano, Wong, Ryan W.K., Mayor, Thibault
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9881406/
https://www.ncbi.nlm.nih.gov/pubmed/36853680
http://dx.doi.org/10.1016/j.xpro.2023.102059

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9881406/
https://www.ncbi.nlm.nih.gov/pubmed/36853680
http://dx.doi.org/10.1016/j.xpro.2023.102059

Ejemplares similares