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Identification of newly translated thermo-sensitive proteins using pulse SILAC mass spectrometry and the GAL promoter system
Some newly translated proteins are more susceptible to misfolding and aggregation upon heat shock in comparison to other proteins. To study these newly translated thermo-sensitive proteins on a proteomic scale, we present here a protocol that combines pulse-SILAC with biochemical fractionation for m...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9881406/ https://www.ncbi.nlm.nih.gov/pubmed/36853680 http://dx.doi.org/10.1016/j.xpro.2023.102059 |