Selection of Extended CRISPR RNAs with Enhanced Targeting and Specificity

For a CRISPR guide RNA (gRNA) with a specific target but activity at known “off-target” sequences, we present a method to screen hundreds of thousands of gRNA variants with short, randomized 5’ nucleotide extensions near its DNA-targeting segment—a modification that can increase Cas9 gene editing sp...

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Detalles Bibliográficos
Autores principales: Herring-Nicholas, Ashley, Dimig, Hillary, Roesing, Miranda, Josephs, Eric A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882064/
https://www.ncbi.nlm.nih.gov/pubmed/36711586
http://dx.doi.org/10.1101/2023.01.11.523593

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882064/
https://www.ncbi.nlm.nih.gov/pubmed/36711586
http://dx.doi.org/10.1101/2023.01.11.523593

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