Improved selection of zebrafish CRISPR editing by early next-generation sequencing based genotyping

Despite numerous prior attempts to improve knock-in (KI) efficiency, the introduction of precise base pair substitutions by the CRISPR-Cas9 technique in zebrafish remains challenging. In our efforts to generate KI zebrafish models of human CACNA1C mutations, we have tested the effect of several CRIS...

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Detalles Bibliográficos
Autores principales: Sieliwonczyk, Ewa, Vandendriessche, Bert, Claes, Charlotte, Mayeur, Evy, Alaerts, Maaike, Holmgren, Philip, Canter Cremers, Tycho, Snyders, Dirk, Loeys, Bart, Schepers, Dorien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9883431/
https://www.ncbi.nlm.nih.gov/pubmed/36707549
http://dx.doi.org/10.1038/s41598-023-27503-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9883431/
https://www.ncbi.nlm.nih.gov/pubmed/36707549
http://dx.doi.org/10.1038/s41598-023-27503-9

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