Cargando…

Improved selection of zebrafish CRISPR editing by early next-generation sequencing based genotyping

Despite numerous prior attempts to improve knock-in (KI) efficiency, the introduction of precise base pair substitutions by the CRISPR-Cas9 technique in zebrafish remains challenging. In our efforts to generate KI zebrafish models of human CACNA1C mutations, we have tested the effect of several CRIS...

Descripción completa

Detalles Bibliográficos
Autores principales:	Sieliwonczyk, Ewa, Vandendriessche, Bert, Claes, Charlotte, Mayeur, Evy, Alaerts, Maaike, Holmgren, Philip, Canter Cremers, Tycho, Snyders, Dirk, Loeys, Bart, Schepers, Dorien
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9883431/ https://www.ncbi.nlm.nih.gov/pubmed/36707549 http://dx.doi.org/10.1038/s41598-023-27503-9

Ejemplares similares

Clinical and functional characterisation of a recurrent KCNQ1 variant in the Belgian population
por: Sieliwonczyk, Ewa, et al.
Publicado: (2023)

Morpho-functional comparison of differentiation protocols to create iPSC-derived cardiomyocytes
por: Nijak, Aleksandra, et al.
Publicado: (2022)

Compound Heterozygous SCN5A Mutations in Severe Sodium Channelopathy With Brugada Syndrome: A Case Report
por: Nijak, Aleksandra, et al.
Publicado: (2020)

iPSC-Cardiomyocyte Models of Brugada Syndrome—Achievements, Challenges and Future Perspectives
por: Nijak, Aleksandra, et al.
Publicado: (2021)

iPSC-Derived Cardiomyocytes in Inherited Cardiac Arrhythmias: Pathomechanistic Discovery and Drug Development
por: Simons, Eline, et al.
Publicado: (2023)

FLNA mutations in surviving males presenting with connective tissue findings: two new case reports and review of the literature
por: Cannaerts, Elyssa, et al.
Publicado: (2018)

Generation of two induced pluripotent stem cell (iPSC) lines (BBANTWi006-A, BBANTWi007-A) from Brugada syndrome patients carrying an SCN5A mutation
por: Simons, Eline, et al.
Publicado: (2022)

Evaluation of CRISPR gene-editing tools in zebrafish
por: Uribe-Salazar, José M., et al.
Publicado: (2022)

The resting membrane potential of hSC-CM in a syncytium is more hyperpolarised than that of isolated cells
por: Van de Sande, Dieter V., et al.
Publicado: (2021)

The Subfamily-Specific Interaction between Kv2.1 and Kv6.4 Subunits Is Determined by Interactions between the N- and C-termini
por: Bocksteins, Elke, et al.
Publicado: (2014)

The Selectivity Filter Is Involved in the U-Type Inactivation Process of Kv2.1 and Kv3.1 Channels
por: Coonen, Laura, et al.
Publicado: (2020)

pBRIT: gene prioritization by correlating functional and phenotypic annotations through integrative data fusion
por: Kumar, Ajay Anand, et al.
Publicado: (2018)

CRISPR prime editing with ribonucleoprotein complexes in zebrafish and primary human cells
por: Petri, Karl, et al.
Publicado: (2022)

Editorial: Next-Generation Sequencing and CRISPR-Cas Editing in Plant Virology
por: Hadidi, Ahmed, et al.
Publicado: (2021)

Precise and efficient genome editing in zebrafish using the CRISPR/Cas9 system
por: Irion, Uwe, et al.
Publicado: (2014)

Heritable and Precise Zebrafish Genome Editing Using a CRISPR-Cas System
por: Hwang, Woong Y., et al.
Publicado: (2013)

Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9
por: Sun, Yonghua, et al.
Publicado: (2020)

Cardiogeneticsbank@UZA: A Collection of DNA, Tissues, and Cell Lines as a Translational Tool
por: Alaerts, Maaike, et al.
Publicado: (2019)

varAmpliCNV: analyzing variance of amplicons to detect CNVs in targeted NGS data
por: Kumar, Ajay Anand, et al.
Publicado: (2022)

Efficient In Vivo Liver-Directed Gene Editing Using CRISPR/Cas9
por: Singh, Kshitiz, et al.
Publicado: (2018)

Engineering the next-generation of CAR T-cells with CRISPR-Cas9 gene editing
por: Dimitri, Alexander, et al.
Publicado: (2022)

Enhancing Understanding of the Visual Cycle by Applying CRISPR/Cas9 Gene Editing in Zebrafish
por: Ward, Rebecca, et al.
Publicado: (2018)

An improved method for precise genome editing in zebrafish using CRISPR-Cas9 technique
por: Gasanov, Eugene V., et al.
Publicado: (2021)

SpG and SpRY variants expand the CRISPR toolbox for genome editing in zebrafish
por: Liang, Fang, et al.
Publicado: (2022)

Educational paper: Connective tissue disorders with vascular involvement: from gene to therapy
por: Van Laer, Lut, et al.
Publicado: (2012)

Next-Generation Sequencing and CRISPR/Cas13 Editing in Viroid Research and Molecular Diagnostics
por: Hadidi, Ahmed
Publicado: (2019)

Expanding CRISPR/Cas9 Genome Editing Capacity in Zebrafish Using SaCas9
por: Feng, Yan, et al.
Publicado: (2016)

Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system
por: Zhang, Yihan, et al.
Publicado: (2017)

Occurrence and Nature of Off-Target Modifications by CRISPR-Cas Genome Editing in Plants
por: Sturme, Mark H. J., et al.
Publicado: (2022)

Effective CRISPR/Cas9-based nucleotide editing in zebrafish to model human genetic cardiovascular disorders
por: Tessadori, Federico, et al.
Publicado: (2018)

Author Correction: SpG and SpRY variants expand the CRISPR toolbox for genome editing in zebrafish
por: Liang, Fang, et al.
Publicado: (2023)

A novel technique based on in vitro oocyte injection to improve CRISPR/Cas9 gene editing in zebrafish
por: Xie, Shao-Lin, et al.
Publicado: (2016)

The Progress of CRISPR/Cas9-Mediated Gene Editing in Generating Mouse/Zebrafish Models of Human Skeletal Diseases
por: Wu, Nan, et al.
Publicado: (2019)

Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos
por: Naert, Thomas, et al.
Publicado: (2020)

CRISPR-Mediated Epigenome Editing
por: Enríquez, Paul
Publicado: (2016)

Editing the microbiome the CRISPR way
por: Ramachandran, Gayetri, et al.
Publicado: (2019)

Analysis of Single Nucleotide Variants in CRISPR-Cas9 Edited Zebrafish Exomes Shows No Evidence of Off-Target Inflation
por: Mooney, Marie R., et al.
Publicado: (2019)

MON-714 CRISPR / Cas9 Genomic Edition of the CDH2 Gene in Zebrafish Leads to Eye and Cardiac Malformation
por: Ventura, Bianca Helena, et al.
Publicado: (2020)

Infertility control of transgenic fluorescent zebrafish with targeted mutagenesis of the dnd1 gene by CRISPR/Cas9 genome editing
por: Chu, Wai-Kwan, et al.
Publicado: (2023)

CRISPR-Cas9 DNA Base-Editing and Prime-Editing
por: Kantor, Ariel, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_rb92ohhi0au11rb7dkovfg9jkt