Direct lysis of 3D cell cultures for RT-qPCR gene expression quantification

In vitro cell culture experiments are widely used to study cellular behavior in most biological research fields. Except for suspension cells, most human cell types are cultured as adherent monolayers on a plastic surface. While technically convenient, monolayer cultures can suffer from limitations i...

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Detalles Bibliográficos
Autores principales: Gysens, Fien, Ostyn, Lisa, Goeteyn, Ellen, Blondeel, Eva, Nuyttens, Justine, De Wever, Olivier, de Bony, Eric, Crabbé, Aurélie, Mestdagh, Pieter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9883454/
https://www.ncbi.nlm.nih.gov/pubmed/36707637
http://dx.doi.org/10.1038/s41598-023-28844-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9883454/
https://www.ncbi.nlm.nih.gov/pubmed/36707637
http://dx.doi.org/10.1038/s41598-023-28844-1

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