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Direct lysis of 3D cell cultures for RT-qPCR gene expression quantification
In vitro cell culture experiments are widely used to study cellular behavior in most biological research fields. Except for suspension cells, most human cell types are cultured as adherent monolayers on a plastic surface. While technically convenient, monolayer cultures can suffer from limitations i...
| Autores principales: | , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9883454/ https://www.ncbi.nlm.nih.gov/pubmed/36707637 http://dx.doi.org/10.1038/s41598-023-28844-1 |