Cargando…
Synaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates
Ectopic expression in fibroblasts of synapsin 1 and synaptophysin is sufficient to generate condensates of vesicles highly reminiscent of synaptic vesicle (SV) clusters and with liquid-like properties. Here we show that unlike synaptophysin, other major integral SV membrane proteins fail to form con...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9884207/ https://www.ncbi.nlm.nih.gov/pubmed/36709207 http://dx.doi.org/10.1038/s41467-023-36081-3 |
_version_ | 1784879667606454272 |
---|---|
author | Park, Daehun Wu, Yumei Wang, Xinbo Gowrishankar, Swetha Baublis, Aaron De Camilli, Pietro |
author_facet | Park, Daehun Wu, Yumei Wang, Xinbo Gowrishankar, Swetha Baublis, Aaron De Camilli, Pietro |
author_sort | Park, Daehun |
collection | PubMed |
description | Ectopic expression in fibroblasts of synapsin 1 and synaptophysin is sufficient to generate condensates of vesicles highly reminiscent of synaptic vesicle (SV) clusters and with liquid-like properties. Here we show that unlike synaptophysin, other major integral SV membrane proteins fail to form condensates with synapsin, but co-assemble into the clusters formed by synaptophysin and synapsin in this ectopic expression system. Another vesicle membrane protein, ATG9A, undergoes activity-dependent exo-endocytosis at synapses, raising questions about the relation of ATG9A traffic to the traffic of SVs. We find that both in fibroblasts and in nerve terminals ATG9A does not co-assemble into synaptophysin-positive vesicle condensates but localizes on a distinct class of vesicles that also assembles with synapsin but into a distinct phase. Our findings suggest that ATG9A undergoes differential sorting relative to SV proteins and also point to a dual role of synapsin in controlling clustering at synapses of SVs and ATG9A vesicles. |
format | Online Article Text |
id | pubmed-9884207 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-98842072023-01-30 Synaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates Park, Daehun Wu, Yumei Wang, Xinbo Gowrishankar, Swetha Baublis, Aaron De Camilli, Pietro Nat Commun Article Ectopic expression in fibroblasts of synapsin 1 and synaptophysin is sufficient to generate condensates of vesicles highly reminiscent of synaptic vesicle (SV) clusters and with liquid-like properties. Here we show that unlike synaptophysin, other major integral SV membrane proteins fail to form condensates with synapsin, but co-assemble into the clusters formed by synaptophysin and synapsin in this ectopic expression system. Another vesicle membrane protein, ATG9A, undergoes activity-dependent exo-endocytosis at synapses, raising questions about the relation of ATG9A traffic to the traffic of SVs. We find that both in fibroblasts and in nerve terminals ATG9A does not co-assemble into synaptophysin-positive vesicle condensates but localizes on a distinct class of vesicles that also assembles with synapsin but into a distinct phase. Our findings suggest that ATG9A undergoes differential sorting relative to SV proteins and also point to a dual role of synapsin in controlling clustering at synapses of SVs and ATG9A vesicles. Nature Publishing Group UK 2023-01-28 /pmc/articles/PMC9884207/ /pubmed/36709207 http://dx.doi.org/10.1038/s41467-023-36081-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Park, Daehun Wu, Yumei Wang, Xinbo Gowrishankar, Swetha Baublis, Aaron De Camilli, Pietro Synaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates |
title | Synaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates |
title_full | Synaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates |
title_fullStr | Synaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates |
title_full_unstemmed | Synaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates |
title_short | Synaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates |
title_sort | synaptic vesicle proteins and atg9a self-organize in distinct vesicle phases within synapsin condensates |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9884207/ https://www.ncbi.nlm.nih.gov/pubmed/36709207 http://dx.doi.org/10.1038/s41467-023-36081-3 |
work_keys_str_mv | AT parkdaehun synapticvesicleproteinsandatg9aselforganizeindistinctvesiclephaseswithinsynapsincondensates AT wuyumei synapticvesicleproteinsandatg9aselforganizeindistinctvesiclephaseswithinsynapsincondensates AT wangxinbo synapticvesicleproteinsandatg9aselforganizeindistinctvesiclephaseswithinsynapsincondensates AT gowrishankarswetha synapticvesicleproteinsandatg9aselforganizeindistinctvesiclephaseswithinsynapsincondensates AT baublisaaron synapticvesicleproteinsandatg9aselforganizeindistinctvesiclephaseswithinsynapsincondensates AT decamillipietro synapticvesicleproteinsandatg9aselforganizeindistinctvesiclephaseswithinsynapsincondensates |