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Tissue libraries enable rapid determination of conditions that preserve antibody labeling in cleared mouse and human tissue

Difficulty achieving complete, specific, and homogenous staining is a major bottleneck preventing the widespread use of tissue clearing techniques to image large volumes of human tissue. In this manuscript, we describe a procedure to rapidly design immunostaining protocols for antibody labeling of c...

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Detalles Bibliográficos
Autores principales: Zwang, Theodore J, Bennett, Rachel E, Lysandrou, Maria, Woost, Benjamin, Zhang, Anqi, Lieber, Charles M, Richardson, Douglas S, Hyman, Bradley T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9889093/
https://www.ncbi.nlm.nih.gov/pubmed/36656755
http://dx.doi.org/10.7554/eLife.84112