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Labeling Cell Surface Receptors with Ligand.BirA* Bispecifics

[Image: see text] BirA*, a mutant form of the biotinylating enzyme BirA, can nonspecifically biotinylate ε-amino groups on lysines of proteins. Based on the promiscuous labeling nature of BirA*, plasmids expressing fusion constructs of BirA* to a given ligand have been used to transfect eukaryotic c...

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Detalles Bibliográficos
Autores principales: Alwash, Mays, Gariépy, Jean
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9890520/
https://www.ncbi.nlm.nih.gov/pubmed/36742360
http://dx.doi.org/10.1021/acsptsci.1c00192