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Labeling Cell Surface Receptors with Ligand.BirA* Bispecifics
[Image: see text] BirA*, a mutant form of the biotinylating enzyme BirA, can nonspecifically biotinylate ε-amino groups on lysines of proteins. Based on the promiscuous labeling nature of BirA*, plasmids expressing fusion constructs of BirA* to a given ligand have been used to transfect eukaryotic c...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9890520/ https://www.ncbi.nlm.nih.gov/pubmed/36742360 http://dx.doi.org/10.1021/acsptsci.1c00192 |