Identification and control for the effects of bioinformatic globin depletion on human RNA-seq differential expression analysis

When profiling blood samples by RNA-sequencing (RNA-seq), RNA from haemoglobin (Hgb) can account for up to 70% of the transcriptome. Due to considerations of sequencing depth and power to detect biological variation, Hgb RNA is typically depleted prior to sequencing by hybridisation-based methods; a...

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Detalles Bibliográficos
Autores principales: Sheerin, Dylan, Lakay, Francisco, Esmail, Hanif, Kinnear, Craig, Sansom, Bianca, Glanzmann, Brigitte, Wilkinson, Robert J., Ritchie, Matthew E., Coussens, Anna K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9892020/
https://www.ncbi.nlm.nih.gov/pubmed/36725870
http://dx.doi.org/10.1038/s41598-023-28218-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9892020/
https://www.ncbi.nlm.nih.gov/pubmed/36725870
http://dx.doi.org/10.1038/s41598-023-28218-7

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