Cargando…
In silico protein engineering shows that novel mutations affecting NAD(+) binding sites may improve phosphite dehydrogenase stability and activity
Pseudomonas stutzeri phosphite dehydrogenase (PTDH) catalyzes the oxidation of phosphite to phosphate in the presence of NAD, resulting in the formation of NADH. The regeneration of NADH by PTDH is greater than any other enzyme due to the substantial change in the free energy of reaction (G°′ = − 63...
| Autores principales: | , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2023
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9892502/ https://www.ncbi.nlm.nih.gov/pubmed/36725973 http://dx.doi.org/10.1038/s41598-023-28246-3 |