Simultaneous N-Deglycosylation and Digestion of Complex Samples on S-Traps Enables Efficient Glycosite Hypothesis Generation

[Image: see text] N-linked glycosylation is an important post-translational modification that is difficult to identify and quantify in traditional bottom-up proteomics experiments. Enzymatic deglycosylation of proteins by peptide:N-glycosidase F (PNGase F) prior to digestion and subsequent mass spec...

Descripción completa

Detalles Bibliográficos
Autores principales: DeRosa, Christine M., Weaver, Simon D., Wang, Chien-Wei, Schuster-Little, Naviya, Whelan, Rebecca J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9893465/
https://www.ncbi.nlm.nih.gov/pubmed/36743002
http://dx.doi.org/10.1021/acsomega.2c08071

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9893465/
https://www.ncbi.nlm.nih.gov/pubmed/36743002
http://dx.doi.org/10.1021/acsomega.2c08071

Ejemplares similares