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Optimization of Degenerate PCR Conditions for Reducing Error Rates in Detection of PKS and NRPS Gene groups in Actinomycetes

BACKGROUND: The screen of Polyketide Synthase (PKS) and Nonribosomal Peptide Synthetase (NRPS) gene groups is a quick way to discover new therapeutic agents. However, errors in laboratory techniques cause a loss of touch with reality. This study aimed to evaluate the presence of PKS and NRPS gene gr...

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Detalles Bibliográficos
Autores principales: Ghashghaei, Sara, Etemadifar, Zahra, Tavassoli, Manoochehr, Mofid, Mohammad Reza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Avicenna Research Institute 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9895980/
https://www.ncbi.nlm.nih.gov/pubmed/36789116
http://dx.doi.org/10.18502/ajmb.v15i1.11422