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Flow cytometry protocol to quantify immune cells in the separated epithelium and stroma of herpes simplex virus-1-infected mouse cornea

Existing flow cytometry approaches identify immune cells using the whole infected/inflamed cornea, which limits its ability to distinguish the immune cells infiltrating the corneal epithelium from the corneal stroma. Here, we present a protocol to analyze immune cells in the separated epithelium and...

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Detalles Bibliográficos
Autores principales: Setia, Mizumi, Suvas, Pratima Krishna, Suvas, Susmit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898781/
https://www.ncbi.nlm.nih.gov/pubmed/36790766
http://dx.doi.org/10.1016/j.xpro.2023.102056