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The pH-responsive precipitation–redissolution of the CspB fusion protein, CspB50TEV-Teriparatide, triggered by changes in secondary structure

Cell surface protein B (CspB) fusion proteins can undergo reversible pH-responsive precipitation–redissolution. A pH-responsive precipitation–redissolution of CspB tag purification (pPRCP) method was established for protein purification using this property. However, the mechanism of the pH-responsiv...

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Detalles Bibliográficos
Autores principales: Nagano, Hayato, Mannen, Teruhisa, Kikuchi, Yoshimi, Shiraki, Kentaro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9900485/
https://www.ncbi.nlm.nih.gov/pubmed/36756166
http://dx.doi.org/10.1016/j.bbrep.2023.101435