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Conformationally responsive dyes enable protein-adaptive differential scanning fluorimetry

Flexible in vitro methods alter the course of biological discoveries. Differential Scanning Fluorimetry (DSF) is a particularly versatile technique which reports protein thermal unfolding via fluorogenic dye. However, applications of DSF are limited by widespread protein incompatibilities with the a...

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Detalles Bibliográficos
Autores principales: Wu, Taiasean, Yu, Joshua C., Suresh, Arundhati, Gale-Day, Zachary J., Alteen, Matthew G., Woo, Amanda S., Millbern, Zoe, Johnson, Oleta T., Carroll, Emma C., Partch, Carrie L., Fourches, Denis, Vinueza, Nelson R., Vocadlo, David J., Gestwicki, Jason E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9900766/
https://www.ncbi.nlm.nih.gov/pubmed/36747624
http://dx.doi.org/10.1101/2023.01.23.525251