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Ultra-Fast Bioorthogonal Spin-Labeling and Distance Measurements in Mammalian Cells Using Small, Genetically Encoded Tetrazine Amino Acids

Studying protein structures and dynamics directly in the cellular environments in which they function is essential to fully understand the molecular mechanisms underlying cellular processes. Site-directed spin-labeling (SDSL)—in combination with double electron–electron resonance (DEER) spectroscopy...

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Detalles Bibliográficos
Autores principales: Jana, Subhashis, Evans, Eric G. B., Jang, Hyo Sang, Zhang, Shuyang, Zhang, Hui, Rajca, Andrzej, Gordon, Sharona E., Zagotta, William N., Stoll, Stefan, Mehl, Ryan A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9901033/
https://www.ncbi.nlm.nih.gov/pubmed/36747808
http://dx.doi.org/10.1101/2023.01.26.525763