Cargando…

Ultra-Fast Bioorthogonal Spin-Labeling and Distance Measurements in Mammalian Cells Using Small, Genetically Encoded Tetrazine Amino Acids

Studying protein structures and dynamics directly in the cellular environments in which they function is essential to fully understand the molecular mechanisms underlying cellular processes. Site-directed spin-labeling (SDSL)—in combination with double electron–electron resonance (DEER) spectroscopy...

Descripción completa

Detalles Bibliográficos
Autores principales:	Jana, Subhashis, Evans, Eric G. B., Jang, Hyo Sang, Zhang, Shuyang, Zhang, Hui, Rajca, Andrzej, Gordon, Sharona E., Zagotta, William N., Stoll, Stefan, Mehl, Ryan A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9901033/ https://www.ncbi.nlm.nih.gov/pubmed/36747808 http://dx.doi.org/10.1101/2023.01.26.525763

Ejemplares similares

Activation and Delivery of Tetrazine-Responsive Bioorthogonal Prodrugs
por: Wang, Yayue, et al.
Publicado: (2020)

Tetrazine bioorthogonal chemistry derived in vivo imaging
por: Zhao, Gaoxiang, et al.
Publicado: (2022)

Nanobody assemblies with fully flexible topology enabled by genetically encoded tetrazine amino acids
por: Van Fossen, Elise M., et al.
Publicado: (2022)

Tetrazine-mediated bioorthogonal prodrug–prodrug activation
por: Neumann, Kevin, et al.
Publicado: (2018)

Versatile naphthalimide tetrazines for fluorogenic bioorthogonal labelling
por: Graziotto, Marcus E., et al.
Publicado: (2021)

Bioorthogonal labeling with tetrazine-dyes for super-resolution microscopy
por: Beliu, Gerti, et al.
Publicado: (2019)

Isonitrile-responsive and bioorthogonally removable tetrazine protecting groups
por: Tu, Julian, et al.
Publicado: (2019)

Concise Synthesis of Functionalized Cyclobutene Analogues for Bioorthogonal Tetrazine Ligation
por: Sun, Jiayu, et al.
Publicado: (2021)

Site-Specific Glycoconjugation of Protein via Bioorthogonal Tetrazine Cycloaddition with a Genetically Encoded trans-Cyclooctene or Bicyclononyne
por: Machida, Takuya, et al.
Publicado: (2015)

Vinylboronic Acids as Efficient Bioorthogonal Reactants for Tetrazine Labeling in Living Cells
por: Eising, Selma, et al.
Publicado: (2018)

Amino acids with fluorescent tetrazine ethers as bioorthogonal handles for peptide modification
por: Ros, Enric, et al.
Publicado: (2022)

Tetrazine-Induced Bioorthogonal Activation of Vitamin E-Modified siRNA for Gene Silencing
por: Zhang, Xueli, et al.
Publicado: (2022)

Highly Stable and Selective Tetrazines for the Coordination-Assisted Bioorthogonal Ligation with Vinylboronic Acids
por: Eising, Selma, et al.
Publicado: (2018)

Tetrazine Carbon Nanotubes for Pretargeted In Vivo “Click‐to‐Release” Bioorthogonal Tumour Imaging
por: Li, He, et al.
Publicado: (2020)

Click to enter: activation of oligo-arginine cell-penetrating peptides by bioorthogonal tetrazine ligations
por: Bode, Saskia A., et al.
Publicado: (2018)

A Systematic Study of Coumarin–Tetrazine Light‐Up Probes for Bioorthogonal Fluorescence Imaging
por: Galeta, Juraj, et al.
Publicado: (2020)

Lipophilicity and Click Reactivity Determine the Performance of Bioorthogonal Tetrazine Tools in Pretargeted In Vivo Chemistry
por: Stéen, E. Johanna L., et al.
Publicado: (2021)

Two‐Factor Fluorogenicity of Tetrazine‐Modified Cyanine‐Styryl Dyes for Bioorthogonal Labelling of DNA
por: Geng, Philipp, et al.
Publicado: (2022)

A benzylic linker promotes methyltransferase catalyzed norbornene transfer for rapid bioorthogonal tetrazine ligation
por: Muttach, F., et al.
Publicado: (2017)

Unraveling Tetrazine-Triggered Bioorthogonal Elimination Enables Chemical Tools for Ultrafast Release and Universal Cleavage
por: Carlson, Jonathan C. T., et al.
Publicado: (2018)

Bioorthogonal Fluorescence Turn‐On Labeling Based on Bicyclononyne−Tetrazine Cycloaddition Reactions that Form Pyridazine Products
por: Siegl, Sebastian J., et al.
Publicado: (2019)

Uncovering the Key Role of Distortion in Bioorthogonal Tetrazine Tools That Defy the Reactivity/Stability Trade-Off
por: Svatunek, Dennis, et al.
Publicado: (2022)

Genetic encoding of a highly photostable, long lifetime fluorescent amino acid for imaging in mammalian cells
por: Jones, Chloe M., et al.
Publicado: (2021)

Cucurbit[7]uril Enhances Distance Measurements of Spin-Labeled Proteins
por: Yang, Zhimin, et al.
Publicado: (2023)

Correction: A benzylic linker promotes methyltransferase catalyzed norbornene transfer for rapid bioorthogonal tetrazine ligation
por: Muttach, F., et al.
Publicado: (2017)

Site-specific bioorthogonal protein labelling by tetrazine ligation using endogenous β-amino acid dienophiles
por: Richter, Daniel, et al.
Publicado: (2023)

Bioorthogonal Tetrazine-Mediated Transfer Reactions Facilitate Reaction Turnover in Nucleic Acid-Templated Detection of MicroRNA
por: Wu, Haoxing, et al.
Publicado: (2014)

Antibody PEGylation in bioorthogonal pretargeting with trans-cyclooctene/tetrazine cycloaddition: in vitro and in vivo evaluation in colorectal cancer models
por: Rondon, Aurélie, et al.
Publicado: (2017)

Direct Cu-mediated aromatic (18)F-labeling of highly reactive tetrazines for pretargeted bioorthogonal PET imaging
por: García-Vázquez, Rocío, et al.
Publicado: (2021)

Tetrazine‐Triggered Bioorthogonal Cleavage of trans‐Cyclooctene‐Caged Phenols Using a Minimal Self‐Immolative Linker Strategy
por: Keppel, Patrick, et al.
Publicado: (2022)

A (99m)Tc-Labelled Tetrazine for Bioorthogonal Chemistry. Synthesis and Biodistribution Studies with Small Molecule trans-Cyclooctene Derivatives
por: Vito, Alyssa, et al.
Publicado: (2016)

Click’n lock: rapid exchange between unsymmetric tetrazines and thiols for reversible, chemoselective functionalisation of biomolecules with on-demand bioorthogonal locking
por: Gavriel, Katerina, et al.
Publicado: (2023)

Photoreactive bioorthogonal lipid probes and their applications in mammalian biology
por: Shanbhag, Karthik, et al.
Publicado: (2022)

Correction: A (99m)Tc-Labelled Tetrazine for Bioorthogonal Chemistry. Synthesis and Biodistribution Studies with Small Molecule trans-Cyclooctene Derivatives
por: Vito, Alyssa, et al.
Publicado: (2017)

Transition metal ion FRET to measure short-range distances at the intracellular surface of the plasma membrane
por: Gordon, Sharona E., et al.
Publicado: (2016)

Long-distance tmFRET using bipyridyl- and phenanthroline-based ligands
por: Gordon, Sharona E., et al.
Publicado: (2023)

(15)N(4)-1,2,4,5-tetrazines as potential molecular tags: Integrating bioorthogonal chemistry with hyperpolarization and unearthing para-N(2)
por: Bae, Junu, et al.
Publicado: (2018)

Live‐Cell Imaging of Sterculic Acid—a Naturally Occurring 1,2‐Cyclopropene Fatty Acid—by Bioorthogonal Reaction with Turn‐On Tetrazine‐Fluorophore Conjugates
por: Bertheussen, Kristine, et al.
Publicado: (2022)

Synthesis and Electron Spin Relaxation of Tetracarboxylate Pyrroline Nitroxides
por: Huang, Shengdian, et al.
Publicado: (2016)

Measuring distances between TRPV1 and the plasma membrane using a noncanonical amino acid and transition metal ion FRET
por: Zagotta, William N., et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_qoofri3kj34oeoqol4n1c4gbmk