pCEC-red: a new vector for easier and faster CRISPR-Cas9 genome editing in Saccharomyces cerevisiae

CRISPR-Cas9 technology is widely used for precise and specific editing of Saccharomyces cerevisiae genome to obtain marker-free engineered hosts. Targeted double-strand breaks are controlled by a guide RNA (gRNA), a chimeric RNA containing a structural segment for Cas9 binding and a 20-mer guide seq...

Descripción completa

Detalles Bibliográficos
Autores principales: Maestroni, Letizia, Butti, Pietro, Senatore, Vittorio Giorgio, Branduardi, Paola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9906608/
https://www.ncbi.nlm.nih.gov/pubmed/36640150
http://dx.doi.org/10.1093/femsyr/foad002

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9906608/
https://www.ncbi.nlm.nih.gov/pubmed/36640150
http://dx.doi.org/10.1093/femsyr/foad002

Ejemplares similares