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pCEC-red: a new vector for easier and faster CRISPR-Cas9 genome editing in Saccharomyces cerevisiae
CRISPR-Cas9 technology is widely used for precise and specific editing of Saccharomyces cerevisiae genome to obtain marker-free engineered hosts. Targeted double-strand breaks are controlled by a guide RNA (gRNA), a chimeric RNA containing a structural segment for Cas9 binding and a 20-mer guide seq...
Autores principales: | Maestroni, Letizia, Butti, Pietro, Senatore, Vittorio Giorgio, Branduardi, Paola |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9906608/ https://www.ncbi.nlm.nih.gov/pubmed/36640150 http://dx.doi.org/10.1093/femsyr/foad002 |
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