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Muscle injury causes long-term changes in stem-cell DNA methylation
Injury to muscle brings about the activation of stem cells, which then generate new myocytes to replace damaged tissue. We demonstrate that this activation is accompanied by a dramatic change in the stem-cell methylation pattern that prepares them epigenetically for terminal myocyte differentiation....
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9907067/ https://www.ncbi.nlm.nih.gov/pubmed/36534800 http://dx.doi.org/10.1073/pnas.2212306119 |
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author | Falick Michaeli, Tal Sabag, Ofra Fok, Rimma Azria, Batia Monin, Jonathan Nevo, Yuval Gielchinsky, Yuval Berman, Benjamin P. Cedar, Howard Bergman, Yehudit |
author_facet | Falick Michaeli, Tal Sabag, Ofra Fok, Rimma Azria, Batia Monin, Jonathan Nevo, Yuval Gielchinsky, Yuval Berman, Benjamin P. Cedar, Howard Bergman, Yehudit |
author_sort | Falick Michaeli, Tal |
collection | PubMed |
description | Injury to muscle brings about the activation of stem cells, which then generate new myocytes to replace damaged tissue. We demonstrate that this activation is accompanied by a dramatic change in the stem-cell methylation pattern that prepares them epigenetically for terminal myocyte differentiation. These de- and de novo methylation events occur at regulatory elements associated with genes involved in myogenesis and are necessary for activation and regeneration. Local injury of one muscle elicits an almost identical epigenetic change in satellite cells from other muscles in the body, in a process mediated by circulating factors. Furthermore, this same methylation state is also generated in muscle stem cells (MuSCs) of female animals following pregnancy, even in the absence of any injury. Unlike the activation-induced expression changes, which are transient, the induced methylation profile is stably maintained in resident MuSCs and thus represents a molecular memory of previous physiological events that is probably programmed to provide a mechanism for long-term adaptation. |
format | Online Article Text |
id | pubmed-9907067 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-99070672023-06-19 Muscle injury causes long-term changes in stem-cell DNA methylation Falick Michaeli, Tal Sabag, Ofra Fok, Rimma Azria, Batia Monin, Jonathan Nevo, Yuval Gielchinsky, Yuval Berman, Benjamin P. Cedar, Howard Bergman, Yehudit Proc Natl Acad Sci U S A Biological Sciences Injury to muscle brings about the activation of stem cells, which then generate new myocytes to replace damaged tissue. We demonstrate that this activation is accompanied by a dramatic change in the stem-cell methylation pattern that prepares them epigenetically for terminal myocyte differentiation. These de- and de novo methylation events occur at regulatory elements associated with genes involved in myogenesis and are necessary for activation and regeneration. Local injury of one muscle elicits an almost identical epigenetic change in satellite cells from other muscles in the body, in a process mediated by circulating factors. Furthermore, this same methylation state is also generated in muscle stem cells (MuSCs) of female animals following pregnancy, even in the absence of any injury. Unlike the activation-induced expression changes, which are transient, the induced methylation profile is stably maintained in resident MuSCs and thus represents a molecular memory of previous physiological events that is probably programmed to provide a mechanism for long-term adaptation. National Academy of Sciences 2022-12-19 2022-12-27 /pmc/articles/PMC9907067/ /pubmed/36534800 http://dx.doi.org/10.1073/pnas.2212306119 Text en Copyright © 2022 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Biological Sciences Falick Michaeli, Tal Sabag, Ofra Fok, Rimma Azria, Batia Monin, Jonathan Nevo, Yuval Gielchinsky, Yuval Berman, Benjamin P. Cedar, Howard Bergman, Yehudit Muscle injury causes long-term changes in stem-cell DNA methylation |
title | Muscle injury causes long-term changes in stem-cell DNA methylation |
title_full | Muscle injury causes long-term changes in stem-cell DNA methylation |
title_fullStr | Muscle injury causes long-term changes in stem-cell DNA methylation |
title_full_unstemmed | Muscle injury causes long-term changes in stem-cell DNA methylation |
title_short | Muscle injury causes long-term changes in stem-cell DNA methylation |
title_sort | muscle injury causes long-term changes in stem-cell dna methylation |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9907067/ https://www.ncbi.nlm.nih.gov/pubmed/36534800 http://dx.doi.org/10.1073/pnas.2212306119 |
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