Systematically attenuating DNA targeting enables CRISPR-driven editing in bacteria

Bacterial genome editing commonly relies on chromosomal cleavage with Cas nucleases to counter-select against unedited cells. However, editing normally requires efficient recombination and high transformation efficiencies, which are unavailable in most strains. Here, we show that systematically atte...

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Detalles Bibliográficos
Autores principales: Collias, Daphne, Vialetto, Elena, Yu, Jiaqi, Co, Khoa, Almási, Éva d. H., Rüttiger, Ann-Sophie, Achmedov, Tatjana, Strowig, Till, Beisel, Chase L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9908933/
https://www.ncbi.nlm.nih.gov/pubmed/36754958
http://dx.doi.org/10.1038/s41467-023-36283-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9908933/
https://www.ncbi.nlm.nih.gov/pubmed/36754958
http://dx.doi.org/10.1038/s41467-023-36283-9

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