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Systematically attenuating DNA targeting enables CRISPR-driven editing in bacteria
Bacterial genome editing commonly relies on chromosomal cleavage with Cas nucleases to counter-select against unedited cells. However, editing normally requires efficient recombination and high transformation efficiencies, which are unavailable in most strains. Here, we show that systematically atte...
| Autores principales: | , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9908933/ https://www.ncbi.nlm.nih.gov/pubmed/36754958 http://dx.doi.org/10.1038/s41467-023-36283-9 |