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ELI trifocal microscope: a precise system to prepare target cryo-lamellae for in situ cryo-ET study

Cryo-electron tomography (cryo-ET) has become a powerful approach to study the high-resolution structure of cellular macromolecular machines in situ. However, the current correlative cryo-fluorescence and electron microscopy lacks sufficient accuracy and efficiency to precisely prepare cryo-lamellae...

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Detalles Bibliográficos
Autores principales: Li, Shuoguo, Wang, Ziyan, Jia, Xing, Niu, Tongxin, Zhang, Jianguo, Yin, Guoliang, Zhang, Xiaoyun, Zhu, Yun, Ji, Gang, Sun, Fei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9911351/
https://www.ncbi.nlm.nih.gov/pubmed/36646897
http://dx.doi.org/10.1038/s41592-022-01748-0