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The PROTECTOR strategy employs dCas orthologs to sterically shield off-target sites from CRISPR/Cas activity
Off-target mutagenesis of CRISPR/Cas systems must be solved to facilitate safe gene therapy. Here, we report a novel approach, termed "PROTECTOR", to shield known off-target sites by directing the binding of an orthologous nuclease-dead Cas protein to the off-target site to sterically inte...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group UK
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9911626/ https://www.ncbi.nlm.nih.gov/pubmed/36759683 http://dx.doi.org/10.1038/s41598-023-29332-2 |