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The PROTECTOR strategy employs dCas orthologs to sterically shield off-target sites from CRISPR/Cas activity

Off-target mutagenesis of CRISPR/Cas systems must be solved to facilitate safe gene therapy. Here, we report a novel approach, termed "PROTECTOR", to shield known off-target sites by directing the binding of an orthologous nuclease-dead Cas protein to the off-target site to sterically inte...

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Detalles Bibliográficos
Autores principales: Sapozhnikov, Daniel M., Szyf, Moshe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9911626/
https://www.ncbi.nlm.nih.gov/pubmed/36759683
http://dx.doi.org/10.1038/s41598-023-29332-2

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