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An Accurate, Rapid and Cost-Effective Method for T-nos Detection Based on CRISPR/Cas12a
CRISPR/Cas12a technology is used for nucleic acid detection due to its specific recognition function and non-specific single-stranded DNA cleavage activity. Here, we developed a fluorescence visualisation detection method based on PCR and CRISPR/Cas12a approaches. The method was used to detect the n...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9914525/ https://www.ncbi.nlm.nih.gov/pubmed/36766144 http://dx.doi.org/10.3390/foods12030615 |