Distinct Mechanistic Behaviour of Tomato CYP74C3 and Maize CYP74A19 Allene Oxide Synthases: Insights from Trapping Experiments and Allene Oxide Isolation

The product specificity and mechanistic peculiarities of two allene oxide synthases, tomato LeAOS3 (CYP74C3) and maize ZmAOS (CYP74A19), were studied. Enzymes were vortexed with linoleic acid 9-hydroperoxide in a hexane–water biphasic system (20–60 s, 0 °C). Synthesized allene oxide (9,10-epoxy-10,1...

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Detalles Bibliográficos
Autores principales: Grechkin, Alexander N., Lantsova, Natalia V., Mukhtarova, Lucia S., Khairutdinov, Bulat I., Gorina, Svetlana S., Iljina, Tatiana M., Toporkova, Yana Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916873/
https://www.ncbi.nlm.nih.gov/pubmed/36768554
http://dx.doi.org/10.3390/ijms24032230

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916873/
https://www.ncbi.nlm.nih.gov/pubmed/36768554
http://dx.doi.org/10.3390/ijms24032230

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