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Single-Molecule Localization Microscopy of Presynaptic Active Zones in Drosophila melanogaster after Rapid Cryofixation

Single-molecule localization microscopy (SMLM) greatly advances structural studies of diverse biological tissues. For example, presynaptic active zone (AZ) nanotopology is resolved in increasing detail. Immunofluorescence imaging of AZ proteins usually relies on epitope preservation using aldehyde-b...

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Detalles Bibliográficos
Autores principales: Mrestani, Achmed, Lichter, Katharina, Sirén, Anna-Leena, Heckmann, Manfred, Paul, Mila M., Pauli, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917252/
https://www.ncbi.nlm.nih.gov/pubmed/36768451
http://dx.doi.org/10.3390/ijms24032128