Efficient bi-allelic tagging in human induced pluripotent stem cells using CRISPR

Allelic tagging of endogenous genes enables studying gene function and transcriptional control in the native genomic context. Here, we present an efficient protocol for bi-allelic tagging of protein-coding genes with fluorescent reporters in human iPSCs using the CRISPR-Cas9-mediated homology-direct...

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Detalles Bibliográficos
Autores principales: Ren, Xingjie, Takagi, Maya Asami, Shen, Yin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9918420/
https://www.ncbi.nlm.nih.gov/pubmed/36853689
http://dx.doi.org/10.1016/j.xpro.2023.102084

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9918420/
https://www.ncbi.nlm.nih.gov/pubmed/36853689
http://dx.doi.org/10.1016/j.xpro.2023.102084

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