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Efficient bi-allelic tagging in human induced pluripotent stem cells using CRISPR
Allelic tagging of endogenous genes enables studying gene function and transcriptional control in the native genomic context. Here, we present an efficient protocol for bi-allelic tagging of protein-coding genes with fluorescent reporters in human iPSCs using the CRISPR-Cas9-mediated homology-direct...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9918420/ https://www.ncbi.nlm.nih.gov/pubmed/36853689 http://dx.doi.org/10.1016/j.xpro.2023.102084 |