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Analyzing mitochondrial respiration of human induced pluripotent stem cell-derived myeloid progenitors using Seahorse technology

Mitochondrial metabolism is critical in hematopoietic stem cell maintenance and differentiation. Here, we present a step-by-step protocol to efficiently differentiate human induced pluripotent stem cells into myeloid progenitors by a robust feeder- and serum-free system. Furthermore, we provide a pr...

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Detalles Bibliográficos
Autores principales: Fan, Yanxin, Mizoguchi, Yoko, Tatematsu, Megumi, Linder, Monika I., Frenz, Stephanie, Choi, Jongsu, Klein, Christoph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9922929/
https://www.ncbi.nlm.nih.gov/pubmed/36853722
http://dx.doi.org/10.1016/j.xpro.2023.102073