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Optimizing DNA Extraction from Pediatric Stool for Diagnosis of Tuberculosis and Use in Next-Generation Sequencing Applications

The WHO has endorsed the use of stool samples for diagnosis of tuberculosis (TB) in children, and targeted next-generation sequencing (tNGS) of stool has been shown to support diagnosis and provide information about drug susceptibility (DS). Optimizing extraction of DNA from stool for sequencing is...

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Detalles Bibliográficos
Autores principales: Ness, Tara E., Meiwes, Lennard, Kay, Alexander, Mejia, Rojelio, Lange, Christoph, Farhat, Maha, Mandalakas, Anna, DiNardo, Andrew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9927083/
https://www.ncbi.nlm.nih.gov/pubmed/36475757
http://dx.doi.org/10.1128/spectrum.02269-22