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RIIβ‐PKA in GABAergic Neurons of Dorsal Median Hypothalamus Governs White Adipose Browning

The RIIβ subunit of  cAMP‐dependent protein kinase A (PKA) is expressed in the brain and adipose tissue. RIIβ‐knockout mice show leanness and increased UCP1 in brown adipose tissue. The authors have previously reported that RIIβ reexpression in hypothalamic GABAergic neurons rescues the leanness. Ho...

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Detalles Bibliográficos
Autores principales: Wang, Bingwei, Zhao, Miao, Su, Zhijie, Jin, Baohua, Yang, Xiaoning, Zhang, Chenyu, Guo, Bingbing, Li, Jiebo, Hong, Weili, Liu, Jiarui, Zhao, Yun, Hou, Yujia, Lai, Futing, Zhang, Wei, Qin, Lihua, Zhang, Weiguang, Luo, Jianyuan, Zheng, Ruimao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9929258/
https://www.ncbi.nlm.nih.gov/pubmed/36529950
http://dx.doi.org/10.1002/advs.202205173
Descripción
Sumario:The RIIβ subunit of  cAMP‐dependent protein kinase A (PKA) is expressed in the brain and adipose tissue. RIIβ‐knockout mice show leanness and increased UCP1 in brown adipose tissue. The authors have previously reported that RIIβ reexpression in hypothalamic GABAergic neurons rescues the leanness. However, whether white adipose tissue (WAT) browning contributes to the leanness and whether RIIβ‐PKA in these neurons governs WAT browning are unknown. Here, this work reports that RIIβ‐KO mice exhibit a robust WAT browning. RIIβ reexpression in dorsal median hypothalamic GABAergic neurons (DMH GABAergic neurons) abrogates WAT browning. Single‐cell sequencing, transcriptome sequencing, and electrophysiological studies show increased GABAergic activity in DMH GABAergic neurons of RIIβ‐KO mice. Activation of DMH GABAergic neurons or inhibition of PKA in these neurons elicits WAT browning and thus lowers body weight. These findings reveal that RIIβ‐PKA in DMH GABAergic neurons regulates WAT browning. Targeting RIIβ‐PKA in DMH GABAergic neurons may offer a clinically useful way to promote WAT browning for treating obesity and other metabolic disorders.