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Protocol for the transformation and engineering of edible algae Arthrospira platensis to generate heterologous protein-expressing strains

Here, we present a protocol for harnessing the natural transformability of the edible algae Arthrospira platensis (common name: spirulina) to generate strains that express heterologous proteins. We describe the preparation of plasmids and the steps to grow A. platensis. We then detail the transforma...

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Detalles Bibliográficos
Autores principales: Tabakh, Hannah, Jester, Benjamin W., Zhao, Hui, Kuestner, Rolf, Khuong, Nhi, Shanitta, Chelsea, Takeuchi, Ryo, Roberts, James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9929480/
https://www.ncbi.nlm.nih.gov/pubmed/36853691
http://dx.doi.org/10.1016/j.xpro.2023.102087