Protocol for the transformation and engineering of edible algae Arthrospira platensis to generate heterologous protein-expressing strains

Here, we present a protocol for harnessing the natural transformability of the edible algae Arthrospira platensis (common name: spirulina) to generate strains that express heterologous proteins. We describe the preparation of plasmids and the steps to grow A. platensis. We then detail the transforma...

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Autores principales: Tabakh, Hannah, Jester, Benjamin W., Zhao, Hui, Kuestner, Rolf, Khuong, Nhi, Shanitta, Chelsea, Takeuchi, Ryo, Roberts, James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9929480/
https://www.ncbi.nlm.nih.gov/pubmed/36853691
http://dx.doi.org/10.1016/j.xpro.2023.102087
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author Tabakh, Hannah
Jester, Benjamin W.
Zhao, Hui
Kuestner, Rolf
Khuong, Nhi
Shanitta, Chelsea
Takeuchi, Ryo
Roberts, James
author_facet Tabakh, Hannah
Jester, Benjamin W.
Zhao, Hui
Kuestner, Rolf
Khuong, Nhi
Shanitta, Chelsea
Takeuchi, Ryo
Roberts, James
author_sort Tabakh, Hannah
collection PubMed
description Here, we present a protocol for harnessing the natural transformability of the edible algae Arthrospira platensis (common name: spirulina) to generate strains that express heterologous proteins. We describe the preparation of plasmids and the steps to grow A. platensis. We then detail the transformation and passage of the strains, followed by genomic DNA extraction and genotyping to assess integration of the gene of interest. This simple transformation protocol can be applied to genome manipulation of edible algae. For complete details on the use and execution of this protocol, please refer to Jester et al. (2022).(1)
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spelling pubmed-99294802023-02-16 Protocol for the transformation and engineering of edible algae Arthrospira platensis to generate heterologous protein-expressing strains Tabakh, Hannah Jester, Benjamin W. Zhao, Hui Kuestner, Rolf Khuong, Nhi Shanitta, Chelsea Takeuchi, Ryo Roberts, James STAR Protoc Protocol Here, we present a protocol for harnessing the natural transformability of the edible algae Arthrospira platensis (common name: spirulina) to generate strains that express heterologous proteins. We describe the preparation of plasmids and the steps to grow A. platensis. We then detail the transformation and passage of the strains, followed by genomic DNA extraction and genotyping to assess integration of the gene of interest. This simple transformation protocol can be applied to genome manipulation of edible algae. For complete details on the use and execution of this protocol, please refer to Jester et al. (2022).(1) Elsevier 2023-02-08 /pmc/articles/PMC9929480/ /pubmed/36853691 http://dx.doi.org/10.1016/j.xpro.2023.102087 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Tabakh, Hannah
Jester, Benjamin W.
Zhao, Hui
Kuestner, Rolf
Khuong, Nhi
Shanitta, Chelsea
Takeuchi, Ryo
Roberts, James
Protocol for the transformation and engineering of edible algae Arthrospira platensis to generate heterologous protein-expressing strains
title Protocol for the transformation and engineering of edible algae Arthrospira platensis to generate heterologous protein-expressing strains
title_full Protocol for the transformation and engineering of edible algae Arthrospira platensis to generate heterologous protein-expressing strains
title_fullStr Protocol for the transformation and engineering of edible algae Arthrospira platensis to generate heterologous protein-expressing strains
title_full_unstemmed Protocol for the transformation and engineering of edible algae Arthrospira platensis to generate heterologous protein-expressing strains
title_short Protocol for the transformation and engineering of edible algae Arthrospira platensis to generate heterologous protein-expressing strains
title_sort protocol for the transformation and engineering of edible algae arthrospira platensis to generate heterologous protein-expressing strains
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9929480/
https://www.ncbi.nlm.nih.gov/pubmed/36853691
http://dx.doi.org/10.1016/j.xpro.2023.102087
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